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Long-term culture-initiating cells (LTC-IC) produced from CD34+ cord blood cells with limiting dilution method
Figure 2. CAFC and CFUs obtained from CD34+ cord blood cells are shown. CAFC were measured at week 5 and then cells were transferred to MethoCult, a semicellulose commercial media used for CFU assays. CFUs were counted on day 12 (week 7). A. Cobblestone Area Forming Cells (CAFCs) after week 5. B. CFU-GEMM: Colony Forming Unit-GranulocyteErythroidMonocyteMacrophage. C. BFU-E: Burst Forming Unit – Erythroid. D. Giemsa-stained BFU-E. E. A HPP-Q (High Proliferative Potential-Quiscent) colony. F. A BFU-E and CFU-GM are seen in a nearby area of the petri dish in MethoCult media
Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo
Figure 1. Micrograph of mesenchymal stem cells maintained in DMEM media for (A) 3 and (B) 5 days
Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo
Figure 2. Micrograph of mesenchymal stem cells after (A) 2nd and(B) 3rd passage
Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo
Figure 3. Micrographs of once-transfected mesenchymal stem cells incubated for (A) 48-, (B) 96-, (C) 144-, and (D) 216 hours p
Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo
Figure 4. Micrographs of twice-transfected mesenchymal stem cells incubated for (A) 48-, (B) 96-, (C) 144- and (D) 216 hours p
A Concise Review on the Classification and Nomenclature of Stem Cells
Figure 1. Differentiation pathways of stem cells
“Niche” concept and the hematopoietic stem cell niches
Figure 1. Two types of niches are defined depending on the existence of parent cells adjacent to stem cell(s). A. Multicellular niche is typically composed of two or more types of parent cells interrelated with each other and with stem cells (arrows). They build several intercellular adhesion sites and molecular signaling pathways providing a suitable niche environment. B. Acellular (extracellular matrix) niche is characterized as the expression of certain cell adhesion molecules on the stem cell surface which interact with specific extracellular matrix proteins (arrows). ECM: Extracellular matrix (original drawing by A. Can).
“Niche” concept and the hematopoietic stem cell niches
Figure 2. N-cadherin (+) cell adhesion sites (small rectangle between osteoblast and hematopoietic stem cell, HSC) stimulate the Wnt/ß-catenin signaling pathway, which induces HSC proliferation while significantly inhibiting their differentiation, thereby resulting in functional selfrenewal (Original drawing by A. Can).
“Niche” concept and the hematopoietic stem cell niches
Figure 3. Notch signaling executed by a series of highly conserved receptors is a key factor in inhibiting differentiation. Intact Notch signaling is required for Wnt-mediated maintenance of undifferentiated HSCs. Notch signaling is active in HSCs and downregulated as HSCs differentiate. Inhibition of Notch signaling leads to accelerated differentiation of HSCs (Original drawing by A. Can).
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